Anti-Human MCP-1/CCL2 Rabbit Recombinant Antibody

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  FC experiment of THP-1 using 98044-1-RR

  1x10^6 untreated or PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.5 ug Anti-Human MCP-1 Rabbit Recombinant Antibody (98044-1-RR, Clone: 230270A7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98044-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98044-1-RR against Human MCP-1/CCL2 were performed. The affinity constant is 8.71 nM.

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98044-1-RR

Clone No.230270A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CCL2, MCP1, MCP-1, CCL-2, CCL 2

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Tested Applications

• Positive FC (Intra) detected in: PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98044-1-RR targets MCP-1/CCL2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC009716
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• RRID: AB_3672191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13500
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.