Anti-Human MCP-1/CCL2 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of THP-1 using 98044-1-RR (same clone as 98044-1-PBS)

  1x10^6 untreated or PMA, LPS and Brefeldin A treated THP-1 cells were intracellularly stained with 0.5 ug Anti-Human MCP-1 Rabbit Recombinant Antibody (98044-1-RR, Clone: 230270A7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98044-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98044-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98044-1-RR against Human MCP-1/CCL2 were performed. The affinity constant is 8.71 nM.

MCP-1/CCL2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98044-1-PBS

Clone No.230270A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CCL2, MCP1, MCP-1, CCL-2, CCL 2

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Product Information

98044-1-PBS targets MCP-1/CCL2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC009716
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13500
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.