MCM6 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MCM6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E12H1

Cat no : 67989-1-PBS

Synonyms

MCG40308, MCM6, Mis5, P105MCM

---

Validation Data Gallery

• 

  WB analysis using 67989-1-Ig (same clone as 67989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67989-1-Ig (MCM6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67989-1-Ig (same clone as 67989-1-PBS)

  WB result of MCM6 antibody (67989-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells. This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67989-1-Ig (same clone as 67989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67989-1-Ig (same clone as 67989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67989-1-Ig (same clone as 67989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67989-1-Ig (same clone as 67989-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 67989-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67989-1-PBS targets MCM6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MCM6 fusion protein Ag4354
• Full Name: minichromosome maintenance complex component 6
• Calculated Molecular Weight: 821 aa, 93 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC032374
• Gene Symbol: MCM6
• Gene ID (NCBI): 4175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.