MCM6 Monoclonal antibody

• Featured Product
• KD/KO Validated

MCM6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12H1

Cat no : 67989-1-Ig

Synonyms

MCG40308, MCM6, Mis5, P105MCM

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Validation Data Gallery

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  WB analysis using 67989-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67989-1-Ig (MCM6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis of HeLa using 67989-1-Ig

  WB result of MCM6 antibody (67989-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

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  IHC staining of human cervical cancer using 67989-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67989-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67989-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of HepG2 using 67989-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, COLO 320 cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67989-1-Ig targets MCM6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MCM6 fusion protein Ag4354
• Full Name: minichromosome maintenance complex component 6
• Calculated Molecular Weight: 821 aa, 93 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC032374
• Gene Symbol: MCM6
• Gene ID (NCBI): 4175
• RRID: AB_2918738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.