CoraLite® Plus 750-conjugated MBP tag Monoclonal antibody

MBP tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4C6H4

Cat no : CL750-66003

Synonyms



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66003 targets MBP tag in WB applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MBP tag fusion protein Ag0942
Full Name MBP tag
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 MBP tag antibody CL750-66003Download protocol
Standard Protocols
Click here to view our Standard Protocols