MBP tag Monoclonal antibody
MBP tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein and More (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
4C6H4
Cat no : 66003-1-Ig
Synonyms
"MBP tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Recombinant protein protein |
| Positive IF/ICC detected in | Transfected cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 37 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 6 publications below |
| CoIP | See 2 publications below |
Product Information
66003-1-Ig targets MBP tag in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MBP tag fusion protein Ag0942 |
| Full Name | MBP tag |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11183040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| IP protocol for MBP tag antibody 66003-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames | ||
Nat Commun Structural insights into pathogenic mechanism of hypohidrotic ectodermal dysplasia caused by ectodysplasin A variants | ||
Nat Commun UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean | ||
Mol Cell Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination. | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Circ Res Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction. |
