CoraLite® Plus 488-conjugated MAPKAPK2 Recombinant antibody

MAPKAPK2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230197D6

Cat no : CL488-82929-2

Synonyms

MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1

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Validation Data Gallery

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  FC experiment of HeLa using CL488-82929-2

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Mapkapk2 Recombinant Antibody (CL488-82929-2, Clone:230197D6)(red), or 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82929-2 is MAPKAPK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAPKAPK2 fusion protein Ag34196
• Full Name: mitogen-activated protein kinase-activated protein kinase 2
• Calculated Molecular Weight: 400 aa, 46 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: BC036060
• Gene Symbol: MAPKAPK2
• Gene ID (NCBI): 9261
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.