MEK1/2 Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 84967-1-RR (same clone as 84967-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84967-1-RR (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01692-1

  Sandwich ELISA standard curve of MP01692-1, Human MEK2 Recombinant Matched Antibody Pair - PBS only. 84967-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1481. 84967-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP01692-1

  The mean MEK2 concentration was determined to be 45.61 ng/mL in A549 cell extract based on a 1.50 mg/mL extract load and 44.07 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

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  IF Staining of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (84967-1-RR, Clone: 242614G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84967-1-RR (same clone as 84967-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MEK1/2 Recombinant Antibody (84967-1-RR, Clone:242614G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84967-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01692-1

  Cytometric bead array standard curve of MP01692-1, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-2-PBS. Detection antibody: 84967-1-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01692-2

  Cytometric bead array standard curve of MP01692-2, MEK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84967-1-PBS. Detection antibody: 84967-3-PBS. Standard: Ag1481. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84967-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84967-1-RR against Human MEK1/2 were performed. The affinity constant is below 1 pM.

MEK1/2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84967-1-PBS

Clone No.242614G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

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Product Information

84967-1-PBS targets MEK1/2 as part of a matched antibody pair:

MP01692-1: 84967-2-PBS capture and 84967-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01692-2: 84967-1-PBS capture and 84967-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MEK1/2 fusion protein Ag1481
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MEK2
• Gene ID (NCBI): 5605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36507
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 84967-1-RR can recognize both the MEK2 and MEK1.