MAP2 Recombinant antibody, PBS Only (Detector)

MAP2 Uni-rAb^TM Recombinant Antibody for IF-P, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241653E9

Cat no : 84306-3-PBS

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01208-3

  Sandwich ELISA standard curve of MP01208-3, Human MAP2 Recombinant Matched Antibody Pair - PBS only. 84306-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11580. 84306-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01208-3

  The mean MAP2 concentration was determined to be 479.14 pg/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load.

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  IF Staining of rat brain using 84306-3-RR (same clone as 84306-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6, green) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).MAP2 antibody (84306-3-RR, Clone: 241653E9,red ) at dilution of 1:210 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 84306-3-RR (same clone as 84306-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using MAP2 antibody (84306-3-RR, Clone: 241653E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01208-1

  Cytometric bead array standard curve of MP01208-1, MAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84306-2-PBS. Detection antibody: 84306-3-PBS. Standard: Ag11580. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01208-2

  Cytometric bead array standard curve of MP01208-2, MAP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84306-1-PBS. Detection antibody: 84306-3-PBS. Standard: Ag11580. Range: 0.625-80 ng/mL.

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Product Information

84306-3-PBS targets MAP2 as part of a matched antibody pair:

MP01208-1: 84306-2-PBS capture and 84306-3-PBS detection (validated in Cytometric bead array)

MP01208-2: 84306-1-PBS capture and 84306-3-PBS detection (validated in Cytometric bead array)

MP01208-3: 84306-5-PBS capture and 84306-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAP2 fusion protein Ag11580
• Full Name: microtubule-associated protein 2
• Calculated Molecular Weight: 200 kDa
• GenBank Accession Number: BC038857
• Gene Symbol: MAP2
• Gene ID (NCBI): 4133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.