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GFAP Recombinant antibody

GFAP Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat , pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4C6

Cat no : 81063-1-RR

Synonyms

FLJ45472, GFAP

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using GFAP Recombinant antibody (81063-1-RR)

    WB analysis of U-251 using 81063-1-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GFAP Recombinant antibody (81063-1-RR)

    WB analysis using 81063-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IHC staining of rat brain using 81063-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of rat brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of mouse brain using 81063-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81063-1-RR

    Affinity and Kinetic Characterization of 81063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inU-251 cells, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4950-1:19800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81063-1-RR targets GFAP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GFAP fusion protein Ag10423
Full Name glial fibrillary acidic protein
Calculated Molecular Weight 432 aa, 50 kDa
GenBank Accession NumberBC013596
Gene Symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

Protocols

Product Specific Protocols
WB protocol for GFAP antibody 81063-1-RRDownload protocol
IHC protocol for GFAP antibody 81063-1-RRDownload protocol
IF protocol for GFAP antibody 81063-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols