CoraLite® Plus 488-conjugated MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12400

Synonyms

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-12400

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MAGEB1 antibody (CL488-12400) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HepG2 using CL488-12400

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Mageb1 Polyclonal Antibody (CL488-12400)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12400 targets MAGEB1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEB1 fusion protein Ag3094
• Full Name: melanoma antigen family B, 1
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC013772
• Gene Symbol: MAGEB1
• Gene ID (NCBI): 4112
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.