MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12400-1-AP

Synonyms

MAGE Xp, MAGE B1 antigen, DSS-AHC critical interval MAGE superfamily 10, DAM10, CT3.1

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Validation Data Gallery

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  WB analysis of HepG2 using 12400-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12400-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12400-1-AP

  IP result of anti-MAGEB1 (IP:12400-1-AP, 4ug; Detection:12400-1-AP 1:2000) with HepG2 cells lysate 3360 ug.

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  IF Staining of HepG2 using 12400-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB1 antibody (12400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A375 using 12400-1-AP

  Immunofluorescent analysis of A375 cells using 12400-1-AP (MAGEB1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, A375 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells, A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12400-1-AP targets MAGEB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEB1 fusion protein Ag3094
• Full Name: melanoma antigen family B, 1
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC013772
• Gene Symbol: MAGEB1
• Gene ID (NCBI): 4112
• RRID: AB_2877854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.