MAEA Recombinant antibody, PBS Only (Capture)

MAEA Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240097E6

Cat no : 83267-4-PBS

Synonyms

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

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Validation Data Gallery

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  WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83267-4-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (83267-4-RR, Clone: 240097E6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-4-RR, Clone:240097E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00233-3

  Cytometric bead array standard curve of MP00233-3, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-4-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

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  Affinity and Kinetic Characterization of 83267-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83267-4-RR against Human MAEA were performed. The affinity constant is 0.283 nM.

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Product Information

83267-4-PBS targets MAEA as part of a matched antibody pair:

MP00233-3: 83267-4-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAEA fusion protein Ag27150
• Full Name: macrophage erythroblast attacher
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001225
• Gene Symbol: MAEA
• Gene ID (NCBI): 10296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).