Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

RB6-8C5

Cat no : 65140-1-Ig

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using 65140-1-Ig

  1X10^6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD11b and 0.5 ug Anti-Mouse Ly-6G/Ly-6C (Gr-1) (65140-1-Ig, Clone:RB6-8C5) and FITC anti-Rat IgG2b Antibody at dilution 1:1000, or 0.5 ug Rat IgG2b isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65140-1-Ig targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Raised against granulocytes of mouse origin
• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly6g
• Gene ID (NCBI): 546644
• RRID: AB_2881475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publications

Species	Application	Title
mouse	FC	J Leukoc Biol; The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis.; Authors - Yitong Liu
mouse	FC	J Oncol; Blocking TGF-β Expression Attenuates Tumor Growth in Lung Cancers, Potentially Mediated by Skewing Development of Neutrophils.; Authors - Yifei Fang