Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa


Mouse and More (2)







Cat no : 65140-1-Ig


Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 2 publications below

Product Information

65140-1-Ig targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivityhuman, mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Raised against granulocytes of mouse origin
Full Name lymphocyte antigen 6 complex, locus G
GenBank Accession NumberX70920
Gene Symbol Ly-6G/Ly-6C
Gene ID (NCBI) 546644
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).


Product Specific Protocols
FC protocol for Ly-6G/Ly-6C (Gr-1) antibody 65140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Leukoc Biol

The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis.

Authors - Yitong Liu

J Oncol

Blocking TGF-β Expression Attenuates Tumor Growth in Lung Cancers, Potentially Mediated by Skewing Development of Neutrophils.

Authors - Yifei Fang

Life Sci

Icariside II alleviates lipopolysaccharide-induced acute lung injury by inhibiting lung epithelial inflammatory and immune responses mediated by neutrophil extracellular traps

Authors - Xiuchun Li

Eur J Pharmacol

Involvement of necroptotic cell death in macrophages in progression of bleomycin and LPS-induced acute exacerbation of idiopathic pulmonary fibrosis

Authors - Atsushi Koike

Sci Rep

Social defeat stress induces liver injury by modulating endoplasmic reticulum stress in C57BL/6J mice

Authors - XiaoLei Gao