CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

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  FC experiment of mouse bone marrow cells using CL647-65140

  1X10^6 mouse bone marrow cells were surface co-stained with FITC Plus Anti-Mouse CD11b and 0.5 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (CL647-65140, Clone: RB6-8C5) or 0.5 ug Control Antibody. Cells were not fixed.

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Cat No. CL647-65140

Clone No.RB6-8C5

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65140 targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Raised against granulocytes of mouse origin

• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly-6G
• Gene ID (NCBI): 546644
• RRID: AB_2883706
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P35461
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).