Mouse Lipocalin-2/Ngal Recombinant antibody, PBS Only (Capture)

Lipocalin-2/Ngal Uni-rAb^TM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230372D6

Cat no : 83102-1-PBS

Synonyms

Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2

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Validation Data Gallery

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  WB analysis using 83102-1-RR (same clone as 83102-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83102-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00302-4

  Sandwich ELISA standard curve of MP00302-4, Mouse NGAL Monoclonal Matched Antibody Pair - PBS only. 83102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0572. 83102-5-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

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  Sample test of MP00302-4

  Urine of eight mice were measured. The NGAL concentration of detected samples was determined to be 63.5 ng/mL with a range of 31.0 - 113.3 ng/mL.

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  Sample test of MP00302-4

  Mouse reticulum cell sarcoma macrophage cells (0.5 x 10^6 cells/mL) were cultured for 1 day in DMEM supplemented with 10% fetal bovine serum containing 20 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of lipopolysaccharide. The mean NGAL concentration was determined to be 2.3 ng/mL in unstimulated J774A.1 supernatant, 248.3 ng/mL in stimulated J774A.1 supernatant.

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  Sample test of MP00302-4

  Serum of sixteen mice were measured. The NGAL concentration of detected samples was determined to be 27.0 ng/mL with a range of 16.3 - 72.7 ng/mL.

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  IHC staining of mouse spleen using 83102-1-RR (same clone as 83102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83102-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83102-1-RR (same clone as 83102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83102-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 83102-1-RR (same clone as 83102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83102-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83102-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83102-1-RR against Mouse Lipocalin-2/Ngal were performed. The affinity constant is below 1 pM.

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Product Information

83102-1-PBS targets Lipocalin-2/Ngal as part of a matched antibody pair:

MP00302-4: 83102-1-PBS capture and 83102-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lipocalin 2
• Calculated Molecular Weight: 23KD
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_008491.1
• Gene Symbol: NGAL
• Gene ID (NCBI): 16819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Ngal.