Lipocalin-2/Ngal Recombinant antibody

Lipocalin-2/Ngal Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230372D6

Cat no : 83102-1-RR

Synonyms

24p3, AW212229, Lcn2, lipocalin 2, NGAL

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Validation Data Gallery

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  WB analysis using 83102-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83102-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83102-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 83102-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83102-1-RR (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83102-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83102-1-RR against Mouse Lipocalin-2/Ngal were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse uterus tissue
• Positive IHC detected in: mouse spleen tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83102-1-RR targets Lipocalin-2/Ngal in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lipocalin 2
• Calculated Molecular Weight: 23KD
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_008491.1
• Gene Symbol: NGAL
• Gene ID (NCBI): 16819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Ngal.