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Mouse NGAL Recombinant antibody, PBS Only (Detector)

NGAL Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230372C6

Cat no : 83102-5-PBS

Synonyms

Lcn2, Lipocalin-2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin, Lipocalin 2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00302-4

    Sandwich ELISA standard curve of MP00302-4

    Sandwich ELISA standard curve of MP00302-4, Mouse NGAL Monoclonal Matched Antibody Pair - PBS only. 83102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0572. 83102-5-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Urine of eight mice were measured. The NGAL concentration of detected samples was determined to be 63.5 ng/mL with a range of 31.0 - 113.3 ng/mL.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Mouse reticulum cell sarcoma macrophage cells (0.5 x 10^6 cells/mL) were cultured for 1 day in DMEM supplemented with 10% fetal bovine serum containing 20 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of lipopolysaccharide. The mean NGAL concentration was determined to be 2.3 ng/mL in unstimulated J774A.1 supernatant, 248.3 ng/mL in stimulated J774A.1 supernatant.

  • Sample test of MP00302-4

    Sample test of MP00302-4

    Serum of sixteen mice were measured. The NGAL concentration of detected samples was determined to be 27.0 ng/mL with a range of 16.3 - 72.7 ng/mL.

  • Cytometric bead array standard curve of MP00302-2

    Cytometric bead array standard curve of MP00302-2

    Cytometric bead array standard curve of MP00302-2, Mouse NGAL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83102-7-PBS. Detection antibody: 83102-5-PBS. Standard: Eg0572. Range: 0.625-20 ng/mL.

Product Information

83102-5-PBS targets NGAL as part of a matched antibody pair:

MP00302-2: 83102-7-PBS capture and 83102-5-PBS detection (validated in Cytometric bead array)

MP00302-4: 83102-1-PBS capture and 83102-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name lipocalin 2
Calculated Molecular Weight23KD
GenBank Accession NumberNM_008491.1
Gene Symbol NGAL
Gene ID (NCBI) 16819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.