CoraLite® Plus 488-conjugated LYN Monoclonal antibody

LYN Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C7F2

Cat no : CL488-60211

Synonyms

Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8, EC:2.7.10.2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-60211

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 LYN antibody (CL488-60211, Clone: 3C7F2 ) at dilution of 1:200.

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  FC experiment of Ramos using CL488-60211

  1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LYN (CL488-60211, Clone:3C7F2) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60211 targets LYN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LYN fusion protein Ag12723
• Full Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Calculated Molecular Weight: 512 aa, 59 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC075002
• Gene Symbol: LYN
• Gene ID (NCBI): 4067
• RRID: AB_2919237
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.