CoraLite® Plus 750-conjugated LY6D Recombinant antibody

LY6D Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4F12

Cat no : CL750-82094

Synonyms

E48, E48 antigen, Ly 6D, LY6D, Lymphocyte antigen 6D

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Validation Data Gallery

• 

  WB analysis using CL750-82094

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82094 (LY6D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82094 targets LY6D in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LY6D fusion protein Ag10714
• Full Name: lymphocyte antigen 6 complex, locus D
• Calculated Molecular Weight: 128 aa, 13 kDa
• Observed Molecular Weight: 13-14 kDa
• GenBank Accession Number: BC031330
• Gene Symbol: LY6D
• Gene ID (NCBI): 8581
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.