LY6D Recombinant antibody

• Featured Product
• KD/KO Validated

LY6D Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F12

Cat no : 82094-1-RR

Synonyms

Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48

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Validation Data Gallery

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  WB analysis of A431 using 82094-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 82094-1-RR (LY6D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 82094-1-RR

  WB result of LY6D antibody (82094-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LY6D transfected A431 cells.

• 

  IHC staining of human skin cancer using 82094-1-RR

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 82094-1-RR

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82094-1-RR targets LY6D in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LY6D fusion protein Ag10714
• Full Name: lymphocyte antigen 6 complex, locus D
• Calculated Molecular Weight: 128 aa, 13 kDa
• Observed Molecular Weight: 13-14 kDa
• GenBank Accession Number: BC031330
• Gene Symbol: LY6D
• Gene ID (NCBI): 8581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.