CoraLite®594-conjugated LRPPRC Monoclonal antibody

LRPPRC Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H7E1

Cat no : CL594-67679

Synonyms

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-67679

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 LRPPRC antibody (CL594-67679, Clone: 3H7E1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67679 targets LRPPRC in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LRPPRC fusion protein Ag30261
• Full Name: leucine-rich PPR-motif containing
• Calculated Molecular Weight: 1394 aa, 158 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC050311
• Gene Symbol: LRPPRC
• Gene ID (NCBI): 10128
• RRID: AB_2920170
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.