LRPPRC Monoclonal antibody

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• KD/KO Validated

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3H7E1

Cat no : 67679-1-Ig

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

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Validation Data Gallery

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  WB analysis using 67679-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67679-1-Ig

  WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells.

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  WB analysis of U-251 using 67679-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67679-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HEK-293 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue).

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  IF Staining of HepG2 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 67679-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, U-251 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells, HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67679-1-Ig targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LRPPRC fusion protein Ag30261
• Full Name: leucine-rich PPR-motif containing
• Calculated Molecular Weight: 1394 aa, 158 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC050311
• Gene Symbol: LRPPRC
• Gene ID (NCBI): 10128
• RRID: AB_2882872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Publications

Species	Application	Title
human	IF	Front Immunol; Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma; Authors - Dongqing Gu
human	WB	Front Genet; RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke; Authors - Kejuan Jia