Filter: AllWBIHCIF/ICC

WB analysis using 67679-1-Ig (same clone as 67679-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)
WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
WB analysis of U-251 using 67679-1-Ig (same clone as 67679-1-PBS)
U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 67679-1-Ig (same clone as 67679-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 67679-1-Ig (same clone as 67679-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

LRPPRC Monoclonal antibody, PBS Only

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67679-1-PBS

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67679-1-PBS targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	LRPPRC fusion protein Ag30261 Predict reactive species
Full Name	leucine-rich PPR-motif containing
Calculated Molecular Weight	1394 aa, 158 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC050311
Gene Symbol	LRPPRC
Gene ID (NCBI)	10128
RRID	AB_2882872
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P42704
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.