PE Anti-Human LRP1 (5A6)

LRP1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

5A6

Cat no : PE-65233

Synonyms

Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor, A2MR

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Validation Data Gallery

• 

  FC experiment of U-87 MG using PE-65233

  1x10^6 U-87 MG cells were surface stained with 5 ul PE Anti-Human LRP1 (PE-65233, Clone:5A6) or PE Mouse IgG2b Isotype Control (PE-65128, Clone: MPC-11). Cells were not fixed.

• 

  FC experiment of U-87 MG using PE-65233

  1x10^6 U-87 MG cells were surface stained with 5 ul PE Anti-Human LRP1 (PE-65233, Clone:5A6) or PE Mouse IgG2b Isotype Control (PE-65128, Clone: MPC-11). Cells were not fixed.

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Tested Applications

• Positive FC detected in: U-87 MG cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65233 targets LRP1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Full length native protein (purified)
• Full Name: low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
• Calculated Molecular Weight: 505 kDa
• GenBank Accession Number: BC045107
• Gene Symbol: LRP1
• Gene ID (NCBI): 4035
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: N/A
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441).