CoraLite®594-conjugated LONP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

LONP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C6C12

Cat no : CL594-66043

Synonyms

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

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Validation Data Gallery

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100. DAPI (blue).

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

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Tested Applications

• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66043 is LONP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LONP1 fusion protein Ag7306
• Full Name: lon peptidase 1, mitochondrial
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000235
• Gene Symbol: LONP1
• Gene ID (NCBI): 9361
• RRID: AB_2919942
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).