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LONP1 Monoclonal antibody
LONP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1C6C12
Cat no : 66043-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | ROS1728 cells, HEK-293 cells, HSC-T6 cells, human heart tissue, human liver tissue, Jurkat cells, L02 cells, RAW 264.7 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C6 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
CoIP | See 1 publications below |
Product Information
66043-1-Ig targets LONP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | LONP1 fusion protein Ag7306 |
Full Name | lon peptidase 1, mitochondrial |
Calculated Molecular Weight | 106 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC000235 |
Gene Symbol | LONP1 |
Gene ID (NCBI) | 9361 |
RRID | AB_2881494 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).
Protocols
Product Specific Protocols | |
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WB protocol for LONP1 antibody 66043-1-Ig | Download protocol |
IHC protocol for LONP1 antibody 66043-1-Ig | Download protocol |
IF protocol for LONP1 antibody 66043-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication | ||
Mol Med ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response | ||
J Physiol Biochem PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle | ||
BMC Neurosci Exercise combined with postbiotics treatment results in synergistic improvement of mitochondrial function in the brain of male transgenic mice for Alzheimer's disease |