LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C12

Cat no : 66043-1-Ig

Synonyms

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15



Tested Applications

Positive WB detected inROS1728 cells, HEK-293 cells, HSC-T6 cells, human heart tissue, human liver tissue, Jurkat cells, L02 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66043-1-Ig is LONP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LONP1 fusion protein Ag7306
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2881494
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

Protocols

Product Specific Protocols
WB protocol for LONP1 antibody 66043-1-IgDownload protocol
IHC protocol for LONP1 antibody 66043-1-IgDownload protocol
IF protocol for LONP1 antibody 66043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanCoIP

Acta Pharm Sin B

Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication

Authors - Xiaowei Wang
humanWB

EMBO J

Stress-dependent macromolecular crowding in the mitochondrial matrix

Authors - Elianne P Bulthuis
human,mouseIHC,WB

Mol Med

ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response

Authors - Yifei Liu
humanWB

Mol Cell

A mitochondrial iron-responsive pathway regulated by DELE1

Authors - Yusuke Sekine
  • KD Validated
human,mouseWB

BMC Neurosci

Exercise combined with postbiotics treatment results in synergistic improvement of mitochondrial function in the brain of male transgenic mice for Alzheimer's disease

Authors - Attila Kolonics