LMOD1 Monoclonal antibody, PBS Only

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  WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60414-1-Ig (same clone as 60414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 60414-1-Ig (same clone as 60414-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using LMOD1 antibody (60414-1-Ig, Clone: 1D6H2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

LMOD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60414-1-PBS

Clone No.1D6H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

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Product Information

60414-1-PBS targets LMOD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LMOD1 fusion protein Ag7568
• Full Name: leiomodin 1 (smooth muscle)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001755
• Gene Symbol: LMOD1
• Gene ID (NCBI): 25802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P29536
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.