LMOD1 Monoclonal antibody

LMOD1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6H2

Cat no : 60414-1-Ig

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

---

Validation Data Gallery

• 

  WB analysis using 60414-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60414-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 60414-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: pig colon tissue, ARPE-19 cells, hTERT-RPE1 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, rabbit testis tissue, rat uterus tissue, mouse uterus tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60414-1-Ig targets LMOD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LMOD1 fusion protein Ag7568
• Full Name: leiomodin 1 (smooth muscle)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001755
• Gene Symbol: LMOD1
• Gene ID (NCBI): 25802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.