LMO7 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84495-4-RR (same clone as 84495-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84495-4-RR (LMO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84495-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01336-4

  Sandwich ELISA standard curve of MP01336-4, Human LMO7 Recombinant Matched Antibody Pair - PBS only. 84495-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30016. 84495-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP01336-4

  The mean LMO7 concentration was determined to be 70.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 99.53 ng/mL in mouse lung tissue extract based on a 1.6 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84495-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84495-4-RR against Human LMO7 were performed. The affinity constant is 0.246 nM.

LMO7 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84495-4-PBS

Clone No.241781D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

LOMP, FBX20, 241781D11

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Product Information

84495-4-PBS targets LMO7 as part of a matched antibody pair:

MP01336-4: 84495-4-PBS capture and 84495-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LMO7 fusion protein Ag30016
• Full Name: LIM domain 7
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 150 kDa, 180 kDa
• GenBank Accession Number: NM_005358
• Gene Symbol: LMO7
• Gene ID (NCBI): 4008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWI1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).