LMO7 Recombinant antibody

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  WB analysis using 84495-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84495-4-RR (LMO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84495-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84495-4-RR against Human LMO7 were performed. The affinity constant is 0.246 nM.

LMO7 Recombinant Antibody for WB, ELISA

Cat No. 84495-4-RR

Clone No.241781D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LOMP, FBX20, 241781D11

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, A549 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84495-4-RR targets LMO7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LMO7 fusion protein Ag30016
• Full Name: LIM domain 7
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 150 kDa, 180 kDa
• GenBank Accession Number: NM_005358
• Gene Symbol: LMO7
• Gene ID (NCBI): 4008
• RRID: AB_3672008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).