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LMO7 Recombinant antibody

LMO7 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241781D11

Cat no : 84495-4-RR

Synonyms

LOMP, FBX20, 241781D11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LMO7 Recombinant antibody (84495-4-RR)

    WB analysis using 84495-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84495-4-RR (LMO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84495-4-RR

    Affinity and Kinetic Characterization of 84495-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84495-4-RR against Human LMO7 were performed. The affinity constant is 0.246 nM.

Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84495-4-RR targets LMO7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LMO7 fusion protein Ag30016
Full Name LIM domain 7
Calculated Molecular Weight 193 kDa
Observed Molecular Weight150 kDa, 180 kDa
GenBank Accession NumberNM_005358
Gene Symbol LMO7
Gene ID (NCBI) 4008
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).

Protocols

Product Specific Protocols
WB protocol for LMO7 antibody 84495-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols