LIMK2 Recombinant antibody

LIMK2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230283D10

Cat no : 82977-1-RR

Synonyms

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

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Validation Data Gallery

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  IF Staining of HepG2 using 82977-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HepG2 using 82977-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LIMK2 Recombinant antibody (82977-1-RR, Clone:230283D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82977-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82977-1-RR targets LIMK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LIMK2 fusion protein Ag3011
• Full Name: LIM domain kinase 2
• Calculated Molecular Weight: 686 aa, 78 kDa
• GenBank Accession Number: BC013051
• Gene Symbol: LIMK2
• Gene ID (NCBI): 3985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.