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CoraLite®647-conjugated LC3 Polyclonal antibody

LC3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®647 Fluorescent Dye

Cat no : CL647-14600

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-14600

    FC experiment of HeLa using CL647-14600

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®647 Anti-Human LC3 (CL647-14600) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14600-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-14600 targets LC3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag6144
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
GenBank accession numberBC067797
Gene symbol MAP1LC3B
Gene ID (NCBI) 81631
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
FC protocol for CL647 LC3 antibody CL647-14600Download protocol
Standard Protocols
Click here to view our Standard Protocols