Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, Chloroquine treated HeLa cells, rat brain tissue |
| Positive IHC detected in | human liver tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1409 publications below |
| IHC | See 160 publications below |
| IF | See 431 publications below |
| IP | See 6 publications below |
| ELISA | See 1 publications below |
| CoIP | See 5 publications below |
Product Information
14600-1-AP targets LC3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, IHC-IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey, chicken, zebrafish, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LC3 fusion protein Ag6144 Predict reactive species |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 14-18 kDa |
| GenBank Accession Number | BC067797 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_2137737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LC3 antibody 14600-1-AP | Download protocol |
| IHC protocol for LC3 antibody 14600-1-AP | Download protocol |
| IF protocol for LC3 antibody 14600-1-AP | Download protocol |
| FC protocol for LC3 antibody 14600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Bioact Mater Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway | ||
Acta Neuropathol C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Aditya (Verified Customer) (11-01-2024) | great with ecl
|
FH David (Verified Customer) (01-02-2024) | Good antibody, single band at the predicted molecular weight, no background.
|
FH Hala (Verified Customer) (05-09-2023) | works very good in milk5%
 |
FH Hala (Verified Customer) (04-28-2023) | works very good with milk blocking
|
FH Zhihao (Verified Customer) (07-25-2022) | We could detect one form of LC3 in our cell samples, which should be two.
 |
FH S (Verified Customer) (12-31-2021) | I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well)
 |
FH YING (Verified Customer) (09-25-2021) | The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A.
 |
FH Eric (Verified Customer) (03-13-2021) | Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.
 |
FH Elzbieta (Verified Customer) (09-23-2020) | The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.
|
FH An (Verified Customer) (09-17-2020) | Used for IHC on zebrafish cryosections of eyes. Worked well.
|
FH David (Verified Customer) (03-30-2020) | Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.
|
