CoraLite® Plus 488-conjugated LAMR1/RPSA Monoclonal antibody

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  IF Staining of A549 using CL488-67324

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LAMR1/RPSA antibody (CL488-67324, Clone: 2A9A1 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-67324

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LAMR1/RPSA (CL488-67324, Clone:2A9A1) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LAMR1/RPSA Monoclonal Antibody for IF/ICC

Cat No. CL488-67324

Clone No.2A9A1

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

LAMR1,RPSA, RPSA, 67LR, 67 kDa laminin receptor, 40S ribosomal protein SA

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67324 targets LAMR1/RPSA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: LAMR1/RPSA fusion protein Ag6190
• Full Name: ribosomal protein SA
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC050688
• Gene Symbol: RPSA
• Gene ID (NCBI): 3921
• RRID: AB_2934536
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• UNIPROT ID: P08865
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.