Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 67324-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67324-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67324-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1/RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1/RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts.
Various lysates were subjected to SDS PAGE followed by western blot with 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 67324-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1/RPSA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67324-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67324-1-Ig (LAMR1,RPSA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 67324-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LAMR1/RPSA antibody (67324-1-Ig, Clone: 2A9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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