Cytokeratin 19 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cytokeratin 19 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G1E4

Cat no : 60187-1-PBS

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Validation Data Gallery

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  WB analysis using 60187-1-Ig (same clone as 60187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60187-1-Ig (same clone as 60187-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60187-1-Ig (same clone as 60187-1-PBS)

  WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 60187-1-Ig (same clone as 60187-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 60187-1-Ig (same clone as 60187-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60187-1-Ig (same clone as 60187-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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Product Information

60187-1-PBS targets Cytokeratin 19 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.