CoraLite® Plus 647-conjugated Cytokeratin 19 Monoclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 19 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL647-60187

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Validation Data Gallery

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  IF Staining of human oesophagus cancer using CL647-60187

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL647-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL647-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-60187

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-60187, Clone:3G1E4) (red), or 0.8 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human breast cancer tissue, human oesophagus cancer tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60187 targets Cytokeratin 19 in IF, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• RRID: AB_2920244
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C