Tested Applications
Positive IF-P detected in | human breast cancer tissue, human oesophagus cancer tissue |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cytokeratin 19 fusion protein Ag7407 Predict reactive species |
Full Name | keratin 19 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC002539 |
Gene Symbol | Cytokeratin 19 |
Gene ID (NCBI) | 3880 |
RRID | AB_2919658 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08727 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
Protocols
Product Specific Protocols | |
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IF protocol for CL555 Cytokeratin 19 antibody CL555-60187 | Download protocol |
FC protocol for CL555 Cytokeratin 19 antibody CL555-60187 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |