CoraLite®555-conjugated Cytokeratin 19 Monoclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 19 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL555-60187

Synonyms

CK19, CK 19, KRT19, Cytokeratin-19, Cytokeratin,Keratin

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Validation Data Gallery

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  IF Staining of human breast cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

• 

  IF Staining of human oesophagus cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

• 

  IF Staining of human oesophagus cancer using CL555-60187

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite®555 Cytokeratin 19 antibody (CL555-60187, Clone: 3G1E4 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL555-60187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 19 (CL555-60187, Clone:3G1E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue, human oesophagus cancer tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60187 targets Cytokeratin 19 in IF-P, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• RRID: AB_2919658
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C