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Cytokeratin 1-specific Recombinant antibody

Cytokeratin 1-specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230261F9

Cat no : 82972-1-RR

Synonyms

Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin, CK-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KRT1-specific Recombinant antibody (82972-1-RR)

    WB analysis using 82972-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82972-1-RR (KRT1-specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 1-specific Recombinant antibody (82972-1-RR)

    FC experiment of A431 using 82972-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT1-specific (82972-1-RR, Clone:230261F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse skin tissue, rat skin tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82972-1-RR targets Cytokeratin 1-specific in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name keratin 1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberNM_006121
Gene Symbol KRT1
Gene ID (NCBI) 3848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 1-specific antibody 82972-1-RRDownload protocol
FC protocol for Cytokeratin 1-specific antibody 82972-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols