KMO Recombinant antibody

KMO Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240586D3

Cat no : 83812-1-RR

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

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Validation Data Gallery

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  WB analysis of MCF-7 using 83812-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 83812-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83812-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83812-1-RR is KMO Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KMO fusion protein Ag35029
• Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC005297
• Gene Symbol: KMO
• Gene ID (NCBI): 8564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).