KMO Recombinant antibody, PBS Only

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  WB analysis of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83812-1-RR (same clone as 83812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83812-1-RR (same clone as 83812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83812-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

KMO {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83812-1-PBS

Clone No.240586D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

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Product Information

83812-1-PBS targets KMO in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KMO fusion protein Ag35029
• Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC005297
• Gene Symbol: KMO
• Gene ID (NCBI): 8564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15229
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).