KMO Recombinant antibody, PBS Only

KMO Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240586D3

Cat no : 83812-1-PBS

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

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Validation Data Gallery

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  WB analysis of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83812-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83812-1-PBS is KMO Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KMO fusion protein Ag35029
• Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Calculated Molecular Weight: 486 aa, 56 kDa
• GenBank Accession Number: BC005297
• Gene Symbol: KMO
• Gene ID (NCBI): 8564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.