KLHDC3 Recombinant antibody, PBS Only (Capture)

KLHDC3 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241475G5

Cat no : 84217-4-PBS

Synonyms

PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5

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Validation Data Gallery

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  WB analysis using 84217-4-RR (same clone as 84217-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 84217-4-RR (same clone as 84217-4-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

• 

  FC experiment of U-251 using 84217-4-RR (same clone as 84217-4-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01151-2

  Cytometric bead array standard curve of MP01151-2, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-2-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01151-3

  Cytometric bead array standard curve of MP01151-3, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-1-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84217-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

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Product Information

84217-4-PBS targets KLHDC3 as part of a matched antibody pair:

MP01151-2: 84217-4-PBS capture and 84217-2-PBS detection (validated in Cytometric bead array)

MP01151-3: 84217-4-PBS capture and 84217-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLHDC3 fusion protein Ag4879
• Full Name: kelch domain containing 3
• Calculated Molecular Weight: 382 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC041793
• Gene Symbol: KLHDC3
• Gene ID (NCBI): 116138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.