KIAA1715 Recombinant antibody, PBS Only

KIAA1715 Uni-rAb^TM Recombinant Antibody for ELISA, IHC, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

240211C7

Cat no : 83418-4-PBS

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

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Validation Data Gallery

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  WB analysis using 83418-4-RR (same clone as 83418-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83418-4-RR (same clone as 83418-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83418-4-RR (same clone as 83418-4-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83418-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

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Product Information

The immunogen of 83418-4-PBS is KIAA1715 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIAA1715 fusion protein Ag34669
• Full Name: KIAA1715
• GenBank Accession Number: BC105132
• Gene Symbol: KIAA1715
• Gene ID (NCBI): 80856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.