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KIAA1715 Recombinant antibody

KIAA1715 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240211C7

Cat no : 83418-4-RR

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Recombinant antibody (83418-4-RR)

    WB analysis using 83418-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Recombinant antibody (83418-4-RR)

    IHC staining of mouse cerebellum using 83418-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of U-251 cells using KIAA1715 Recombinant antibody (83418-4-RR)

    FC experiment of U-251 using 83418-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83418-4-RR

    Affinity and Kinetic Characterization of 83418-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

Tested Applications

Positive WB detected inmouse testis tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83418-4-RR is KIAA1715 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669
Full Name KIAA1715
Observed Molecular Weight51 kDa
GenBank Accession NumberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-4-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols