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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21129

Synonyms

CEMIP, hybid, KIAA1199, Protein KIAA1199, TMEM2L

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated KIAA1199 / CEMIP Polyclon (CL594-21129)

    FC experiment of HepG2 using CL594-21129

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KIAA1199 (CL594-21129) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-21129 targets KIAA1199 / CEMIP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1199 / CEMIP fusion protein Ag15527
Full Name KIAA1199
Calculated Molecular Weight 1361 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC020256
Gene Symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_2919859
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein with genetic mutations linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Protocols

Product Specific Protocols
FC protocol for CL594 KIAA1199 / CEMIP antibody CL594-21129Download protocol
Standard Protocols
Click here to view our Standard Protocols