CoraLite®594-conjugated KIAA1199 / CEMIP Polyclonal antibody

• Featured Product
• KD/KO Validated

KIAA1199 / CEMIP Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21129

Synonyms

CEMIP, hybid, KIAA1199, Protein KIAA1199, TMEM2L

---

Validation Data Gallery

• 

  FC experiment of HepG2 using CL594-21129

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KIAA1199 (CL594-21129) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-21129 targets KIAA1199 / CEMIP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA1199 / CEMIP fusion protein Ag15527
• Full Name: KIAA1199 / CEMIP
• Calculated Molecular Weight: 1361 aa, 153 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC020256
• Gene Symbol: KIAA1199
• Gene ID (NCBI): 57214
• RRID: AB_2919859
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein with genetic mutations linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C