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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21129

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated KIAA1199 / CEMIP Po (CL488-21129)

    IF Staining of HeLa using CL488-21129

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIAA1199 / CEMIP antibody (CL488-21129) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated KIAA1199 / CEMIP Po (CL488-21129)

    FC experiment of HepG2 using CL488-21129

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human KIAA1199 / CEMIP (CL488-21129) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21129 targets KIAA1199 / CEMIP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1199 / CEMIP fusion protein Ag15527
Full Name KIAA1199
Calculated Molecular Weight 1361 aa, 153 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC020256
Gene Symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_2919177
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KIAA1199 / CEMIP antibody CL488-21129Download protocol
Standard Protocols
Click here to view our Standard Protocols