CoraLite®594-conjugated KI67 Polyclonal antibody

• Featured Product
• KD/KO Validated

KI67 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-27309

Synonyms

Antigen KI 67, Ki 67, KI67, KIA, MKI67

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Validation Data Gallery

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  IF Staining of HeLa using CL594-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

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  FC experiment of HeLa using CL594-27309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KI67 (CL594-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Ramos using CL594-27309

  1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human KI67 (CL594-27309)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Ramos cells, HeLa cells
• Positive FC detected in: Ramos cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-27309 targets KI67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KI67 fusion protein Ag26266
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• RRID: AB_2919880
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
human	IF	Oncol Rep; Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer; Authors - Liang Xu