Ki-67 Polyclonal antibody

• Featured Product
• KD/KO Validated

Ki-67 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

IHC, IF/ICC, FC (Intra), ELISA, Cell treatment

Conjugate

Unconjugated

Publications

1079

Reviews (5)

Cat no : 27309-1-AP

Synonyms

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

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Validation Data Gallery

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  IHC staining of human tonsillitis using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of K-562 using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded k562 slide using 27309-1-AP (KI67 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (27309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27309-1-AP (KI67 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

• 

  IF Staining of HEK-293 using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using KI67 antibody (27309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (27309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of Jurkat using 27309-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human KI67 (27309-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, K-562 cells, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue, human lymphoma tissue, human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 824 publications below
• IF: See 198 publications below
• FC: See 1 publications below

Product Information

27309-1-AP targets Ki-67 in IHC, IF/ICC, FC (Intra), ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit, canine, hamster, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ki-67 fusion protein Ag26266
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• RRID: AB_2756525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
	IHC	Signal Transduct Target Ther; FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth; Authors - Wenxia Wei
	IHC	Mol Cancer; lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2; Authors - Jin Wang
	IHC	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	IHC	Cancer Discov; Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.; Authors - Guillaume Fonteneau
	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
	IHC	Protein Cell; NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferation; Authors - Yirui Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

Clear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: ARPE-19

Ki-67 Antibody Immunofluorescence validation (1:200 dilution) in ARPE-19 (Cat no:27309-1-AP)

FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: ARPE-19

FH

Ana (Verified Customer) (02-15-2022)

Both, 1:50 and 1:100 dilutions worked well

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50, 1:100
• Cell Tissue Type: ARPE-19

FH

kes (Verified Customer) (01-17-2022)

Works at 1:200 dilution for IHC

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: murine tissues

FH

Yan (Verified Customer) (02-18-2020)

1:100 work for cell.

• Applications: Immunofluorescence,

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