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  • KD/KO Validated

KI67 Polyclonal antibody

KI67 Polyclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)





Cat no : 27309-1-AP


Antigen KI 67, Ki 67, KI67, KIA, MKI67

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, K-562 cells, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue, human lymphoma tissue, human colon cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells
Positive FC detected inJurkat cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27309-1-AP targets KI67 in IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat, rabbit, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KI67 fusion protein Ag26266
Full Name antigen identified by monoclonal antibody Ki-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol MKI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.


Product Specific Protocols
IHC protocol for KI67 antibody 27309-1-APDownload protocol
IF protocol for KI67 antibody 27309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei

Mol Cancer

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

Authors - Jin Wang

Cancer Discov

Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.

Authors - Guillaume Fonteneau


Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.

Authors - Yuhong Luo

ACS Nano

Enzyme-Triggered Transcytosis of Dendrimer-Drug Conjugate for Deep Penetration into Pancreatic Tumors.

Authors - Guowei Wang


C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Authors - Li-Xue Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guo-rong (Verified Customer) (08-30-2022)

Clear staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: ARPE-19
KI67 Antibody Immunofluorescence validation (1:200 dilution) in ARPE-19 (Cat no:27309-1-AP)

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: ARPE-19

Ana (Verified Customer) (02-15-2022)

Both, 1:50 and 1:100 dilutions worked well

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50, 1:100
  • Cell Tissue Type: ARPE-19

kes (Verified Customer) (01-17-2022)

Works at 1:200 dilution for IHC

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: murine tissues

Yan (Verified Customer) (02-18-2020)

1:100 work for cell.

  • Applications: Immunofluorescence,